BaP BaP-induced hepatocarcinogenesis

finalized

Polycyclic aromatic hydrocarbons (PAHs) constitute a large class of ubiquitous environmental pollutants formed after incomplete combustion of organic substances, such as coal, wood, charbroiled meat and at high levels in tobacco smoke. Benzo(a)pyrene (BaP), a representative PAH with potent carcinogenic properties, presents several biological and mutagenic effects, including DNA damage, reactive oxygen species (ROS) formation and alteration of transcription factor networks dynamics.

Hepatocellular carcinoma (HCC) accounts for 70-85% of all primary liver cancers worldwide, being regarded as one of the most lethal type of cancer due to its low survival rate. Although chronic inflammation due to viral infection with HB(C)V or alcohol consumption pose as the main risk factors, tobacco smoking is arising as a major risk factor for disease development.

Thus, this project aims to underline important transcriptomic alterations in in vitro hepatic models exposed to BaP and/or its main metabolites that may influence development and/or progression of HCC into a more malignant phenotype. The study will focus on results obtained from high throughput techniques (microarrays and RNA-seq) from different cell models (HepaRG, HepG2 and primary human hepatocytes) in order to identify important pathways and transcription factor networks related to the onset of BaP-induced hepatocarcinogenesis

Associated Staff

Prof Jos CS Kleinjans PhD
Professor

Prof. Kleinjans studied biology at the Catholic University of Nijmegen and took a training in general biology and further trainings in physiological p more ...

Danyel GJ Jennen PhD
Assistant Professor

Dr. Danyel Jennen is Assistant Professor in the field of toxicoinformatics at the UM Department of Toxicogenomics and he heads its core unit on toxico more ...

Terezinha M de Souza MSc
PhD Student

Terezinha Souza was born on December 7th 1988 in Fortaleza, Brazil. In 2007 she started her Bachelor studies in Biological Sciences at Federal Univers more ...